Correction data.P75 - SRA

SRX8827855: Correction data.P75
1 ILLUMINA (Illumina NovaSeq 6000) run: 25M spots, 7.5G bases, 2.1Gb downloads

Design: Lungfish gDNA for genome correction was isolated by standard gDNA isolation protocol. brief, snap frozen muscle tissue (0.3 g) was powdered and transferred into SDS buffer with Proteinase K (10 _g/ml) and incubated overnight at 65_. DNA solution was purified by two sequential phenol/chloroform extractions. After DNA precipitation, DNA pellet was re613 suspended in 1 ml of Elution Buffer (QIAGEN). RNase A treatment was carried out for 1h at 37C. The DNA solution was purified by two sequential phenol/chloroform extractions and DNA precipitation. gDNA was re-suspended in 100_l of TE buffer. Library preparation was performed using the Westburg NGS DNA library kit. The final library was excised by the Pippin prep with 400bp DNA size.

Submitted by: University of Konstanz

Study: Neoceratodus forsteri isolate:LF-2020 Genome sequencing and assembly

PRJNA644903 • SRP273633 • All experiments • All runs

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Chromosome-scale Hi-C assembly of the Australian lungfish

Sample: Genome correction

SAMN15646770 • SRS7092320 • All experiments • All runs

Organism: Neoceratodus forsteri

Library:

Name: NeoFor_correction_part75

Instrument: Illumina NovaSeq 6000

Strategy: WGS